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Isotopic traceability in body tissues and in different breeding periods of broilers Arq. Bras. Med. Vet. Zootec.
Oliveira,R.P.; Ducatti,C.; Carrijo,A.S.; Pezzato,A.C.; Denadai,J.C.; Sartori,J.R.; Cruz,V.C..
The inclusion of poultry viscera meal (VM) in broiler diets to possibly replace it with strictly vegetable diet (VE) and vice-versa, using the technique of carbon and nitrogen stable isotopes in different tissues was traced. One hundred ninety-two one-day-old chicks that had been distributed randomly were used in twelve experimental treatments with four replications of four chicks each. The treatments consisted of vegetable diet (VG) for diets containing FV going after a certain age or the reverse, in which the birds began feeding VM on diet and then switched to VE diet. At 42 days of age, samples of pectoral muscle (Pectoralis major), keel and tibia were collected, with later determination of isotopic ratios (13C/12C and 15N/14N). The stable isotopes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Poultry viscera meal; Stable isotopes; Carbon-13; Nitrogen-15.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500033
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Multivariate analysis of stable isotope data in the traceability process for birds - doi: 10.4025/actascianimsci.v34i4.13434 Animal Sciences
Sartori, Maria Márcia Pereira; Denadai, Juliana Célia; Garcia, Amélia Maria Lima; Carrijo, Alfredo Sampaio; Ducatti, Carlos.
 Isotope analysis has proved to be an extremely important tool in the traceability process; however, statistical analyses of the results show discrepancies, as the data depend on and originate from several chemical elements such as carbon, hydrogen, oxygen, nitrogen and sulfur (CHONS). In order to establish the proper analysis of traceability data for birds using the stable isotope technique and evaluate the need for a combined analysis of the variables, data for carbon-13 and nitrogen-15 were used from eggs (albumen + yolk) of laying hens and the pectoral muscle of broilers, which were subjected to univariate statistical analysis (ANOVA and complemented with Tukey’s test) and multivariate statistical analysis (MANOVA and Discriminant Analysis). The data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.00.00-2 ANOVA; Discriminant analysis; Carbon-13; MANOVA; Nitrogen-15.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/13434
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Nitrogen mineralization in soils amended with sunnhemp, velvet bean and common bean residues Scientia Agricola
Ambrosano,Edmilson José; Trivelin,Paulo Cesar Ocheuze; Cantarella,Heitor; Ambrosano,Gláucia Maria Bovi; Muraoka,Takashi.
Nitrogen (15N) released from sunnhemp (Crotalaria juncea), velvet bean (Mucuna aterrima) and from Phaseolus bean residues was evaluated after incubation of the plant material in an Eutrudox and a Paleudalf, in a greenhouse experiment with pots containing 6 kg of air dried soil. Dry matter equivalent to 13 Mg ha-1 of Phaseolus bean residues and the same amount of above ground parts of the leguminous species, associated to 2.7 and 2.2 Mg ha-1 of roots of sunnhemp and velvet bean respectively, were incorporated into the soil. A completely randomized experimental design was adopted, with treatments arranged in a 2 <FONT FACE=Symbol>´</FONT> 3 + 1 factorial, replicated three times. The treatments were the following: two soils (Eutrudox and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mineral nitrogen; Green manure; Nitrogen-15; N-mineralization.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100020
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Rastreabilidade de subprodutos de origem animal em dietas com levedura e trigo para frangos PAB
Gottmann,Rosana; Pezzato,Antonio Celso; Ducatti,Carlos; Denadai,Juliana Célia; Móri,Cleusa; Mituo,Mariela Akie Okino; Sartori,José Roberto.
O objetivo deste trabalho foi rastrear a inclussão de farinhas de origem animal em rações para frango de corte com ou sem levedura de cana-de-açúcar e farelo de trigo, por meio da análise do músculo peitoral das aves pelas técnicas dos isótopos estáveis de carbono e nitrogênio. Foram utilizados 210 pintos machos (Cobb), com um dia de idade, distribuídos aleatoriamente em sete tratamentos de 30 aves cada, tendo sido um tratamento controle (dieta vegetal) e seis com inclusão de farinha de carne e ossos bovina ou farinha de vísceras de aves na dieta, com ou sem levedura de cana-de-açúcar e farelo de trigo. Aos 42 dias de idade, foram abatidas quatro aves, por tratamento, escolhidas ao acaso, cujo músculo peitoral foi retirado para análise da razão isotópica....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbon-13; Certification; Meat and bones meal; Poultry offal meal; Nitrogen-15.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001200001
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Rastreability of bovine meat and bone meal using the stable isotope technique in egg production Scientia Agricola
Denadai,Juliana Célia; Batistioli,Julianna Santos; Sartori,José Roberto; Madeira,Luciene Aparecida; Sartori,Maria Márcia Pereira; Pizzolante,Carla Cachoni; Kakimoto,Sérgio Kenji; Contin Neto,Armando Carlos; Costa,Vladimir Eliodoro.
ABSTRACT The present study aimed to verify whether the inclusion of other ingredients in the diet of laying hens could interfere with the traceability of bovine meat and bone meal (BMBM) in eggs through stable carbon and nitrogen isotope analysis. Two hundred and fifty-six white laying hens were used and randomly distributed into eight treatments composed of: CONTROL: control diet; GLUTEN: control diet + corn gluten; YEAST: control diet + yeast; GLUTEN + YEAST: control diet + corn gluten + yeast; BMBM: control diet + 4.5 % BMBM; BMBM + GLUTEN: control diet + corn gluten + 4.5 % BMBM; BMBM + YEAST: control diet + yeast + 4.5 % BMBM; BMBM + GLUTEN + YEAST: control diet + corn gluten + yeast + 4.5 % BMBM. The isotopic results were subjected to multivariate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Albumen; Carbon-13; Nitrogen-15; Traceability; Yolk.
Ano: 2022 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000100402
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Standardization of A Sample-Processing Methodology for Stable Isotope Studies in Poultry Rev. Bras. Ciênc. Avic.
Denadai,JC; Sartori,JB; Pezzato,AC; Costa,VE; Sartori,MMP; Petinati,BES; Gennari,RS; Silva,ET; Carvalho,MAG; Ishizuka,AND.
ABSTRACT The objective of this study was to determine if lipid extraction processes alter the isotopic value of δ13C and δ15N of tissues (pectoral muscle, thigh and liver) and eggs and if the use of anticoagulants interferes with blood and plasma δ13C and δ15N isotopic values. Samples were acquired from the same flock of birds. The 32 egg samples were randomly divided into four treatments (liquid, dehydrated, and fat-extracted with ether or chloroform + methanol) with eight replicates each. The 24 samples of pectoral muscle, thigh muscle and liver of broilers were randomly divided into three treatments (dehydrated, fat-extracted with ether and chloroform + methanol) with eight replicates each. Blood samples were divided into a 3x3 factorial arrangement...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbon-13; Broilers; Nitrogen-15; Laying hens.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100302
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The use of carbon and nitrogen stable isotopes for the detection of poultry offal meal in meat-type quail feeds Rev. Bras. Ciênc. Avic.
Sernagiotto,ER; Ducatti,C; Sartori,JR; Stradiotti,AC; Maruno,MK; Araujo,PC; Carvalho,FB; Pezzato,AC.
The objective of the present study was to trace the inclusion of poultry offal meal (POM) in the diet of meat-type quails reared for a long period using the technique of stable isotopes. A number of 320 quails were randomly distributed into eight treatments: vegetable diet (T1), and a diet containing 8% POM were fed until the end of the experimental period (T2) or replaced by the vegetable diet on day 42 (T3), 56 (T4), 70 (T5), 84 (T6), 98 (T7), and 112 (T8). Breast muscle samples were collected from four birds randomly selected per treatment every 14 days. The obtained isotope results were submitted to multivariate analysis of variance (MANOVA) with the aid of the GLM procedure of statistical SAS program. Treatments were different from T1 when birds were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbon-13; Quail production; Stable isotopes; Nitrogen-15; Traceability.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000100011
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Traceability of animal meals in Japanese quail eggs using the technique of 13C e 15N* stable isotopes Rev. Bras. Ciênc. Avic.
Mori,C; Ducatti,C; Pizzolante,CC; Kakimoto,SK; Denadai,JC.
The objective of this study was to trace the inclusion of bovine meat and bone meal (BMBM) in the diet of Japanese quails by analyzing eggs and egg fractions (yolk and albumen) by the technique of carbon-13 (13C) and nitrogen-15 (15N) stable isotopes. In the trial, 120 Japanese quails were distributed in six treatments with four replicates of five birds each. The following treatments were applied: feed based on corn and soybean meal, containing graded BMBM inclusions (0, 1, 2, 3, 4 or 5%). After 42 days, 20 eggs per treatment were randomly collected for three consecutive days. Ten eggs were used for yolk and albumen sample collection, and ten for total egg sample collection. It was possible to detect the dietary inclusion of 1% BMBM in the egg and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbon-13; Coturnix coturnix japonica; Bovine meat and bone meal; Certification; Nitrogen-15.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000100010
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Traceability of bovine meat and bone meal in poultry by stable isotope analysis Rev. Bras. Ciênc. Avic.
Carrijo,AS; Pezzato,AC; Ducatti,C; Sartori,JR; Trinca,L; Silva,ET.
Bovine meat and bone meal (MBM) was widely used in animal diets until outbreaks of Bovine Spongiform Encefalopathy (BSE) occurred in some countries. It has not been confirmed yet whether or not BSE may be transmitted to man through chicken meat originated from poultry that had been fed diets containing MBM. Therefore, consumers nowadays express preference for meat originated from birds fed exclusively vegetable diets. This study analyzed samples of major breast muscle (Pectoralis major) using mass spectrometry of stable isotopes (carbon and nitrogen) as a means to assess the presence of MBM in broiler diets, a technique that might be used in the certification of poultry quality. A total of 150 day-old chicks were reared in five randomized treatments with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Authentication; Carbon-13; Isotopic determination; Nitrogen-15; Tracer.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100010
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Traceability of poultry offal meal in broiler feeding using isotopic analysis (δ13C and δ15N) of different tissues Rev. Bras. Ciênc. Avic.
Oliveira,RP; Ducatti,C; Pezzato,AC; Denadai,JC; Cruz,VC; Sartori,JR; Carrijo,AS; Caldara,FR.
Our goal was to trace the inclusion of poultry offal meal (OM) in diets by using carbon (13C/12C) and nitrogen (15N/14N) isotopic ratios of different tissues in order to contribute for the development of an independent technology for the certification of the feeding of broilers reared on diets with no addition of animal ingredients. Eighty one-day-old chicks were randomly distributed into five experimental treatments, that is, diets containing increasing levels of OM inclusion (0, 2, 4, 8 and 16% OM), with four replicates of four birds each. At 42 days of age, four birds per treatment (n=4) were randomly selected, weighed, and sacrificed to collect breast muscle (Pectoralis major), keel and tibia samples to determine their isotopic ratios (13C/12C e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Carbon-13; Certification; Feeding regime; Nitrogen-15.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000100002
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